Melamine contamination in feed and raw milk poses severe risks to animal health and food safety, as its high nitrogen content (66.7%) is illegally used to falsify crude protein levels. Traditional detection methods often involve toxic ion-pair reagents that damage chromatography columns and require frequent mobile phase changes. This study introduces a high-performance liquid chromatography (HPLC) method optimised from GB/T 22400-2008, enabling accurate melamine quantification in feed without column or mobile phase replacement. With a detection limit of 2.0 mg·kg⁻¹ and recoveries of 78.8–80.1%, this method is ideal for routine feed quality control, inspection laboratories, and feed manufacturers.
| Item | Proposed HPLC Method | NY/T 1372-2007 Standard |
| Reagents | No ion-pair reagents | Uses toxic ion-pair reagents |
| Column Life | Long (no corrosion) | Short (easy damage) |
| Mobile Phase | Stable, no frequent changes | Requires frequent replacement |
| Operation | Simple, fast | Complex, time-consuming |
| Detection Limit | 2.0 mg·kg⁻¹ | Meets regulatory limits |
| Recovery | 78.8–80.1% | Similar accuracy |
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